Precision-Cut Liver Slices as a New Model to Study Toxicity-Induced Hepatic Stellate Cell Activation in a Physiologic Milieu
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منابع مشابه
Precision-cut liver slices as a new model to study toxicity-induced hepatic stellate cell activation in a physiologic milieu.
Hepatic stellate cell (HSC) activation is a key event in the natural process of wound healing as well as in fibrosis development in liver. Current in vitro models for HSC activation contribute significantly to the understanding of HSC biology and fibrogenesis but still fall far short of recapitulating in vivo intercellular functional and anatomic relationships. In addition, when cultured on unc...
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Introduction Hepatic macrophages have the capacity to secrete a tremendous array of molecules, which can be divided into 3 categories – cytokines (TNF-alpha), lipid mediators (prostaglandins PGE2) and reactive intermediates (NO·) – in response to stimulus, such as lipopolysaccharides (LPS) [1,2]. Such mediators are capable to modulate both the metabolism and the integrity of hepatocytes in vitr...
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Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used therapeutic agents, however, they are associated with a high prevalence of intestinal side effects. In this investigation, rat precision cut intestinal slices (PCIS) were evaluated as an ex vivo model to study NSAID-induced intestinal toxicity. Firstly, PCIS were incubated with 0-200 μM diclofenac (DCF), one of the most intensively ...
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ژورنال
عنوان ژورنال: Toxicological Sciences
سال: 2005
ISSN: 1096-0929,1096-6080
DOI: 10.1093/toxsci/kfi127